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Part:BBa_M30100:Design

Designed by: Dawn Spelke   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


Dspelke: M13.1 - Refactored Genome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1482
    Illegal XbaI site found at 1989
    Illegal SpeI site found at 1745
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1482
    Illegal NheI site found at 1196
    Illegal NheI site found at 1618
    Illegal SpeI site found at 1745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1482
    Illegal XbaI site found at 1989
    Illegal SpeI site found at 1745
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1482
    Illegal XbaI site found at 1989
    Illegal SpeI site found at 1745
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Refactored to make phage more conducive to future engineering attempts, as well as to attempt some more interesting modifications.


Source

M13K07 sequence

References